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SRX20503020: RNA-seq, P. furiosus COM1 WT strain, replicate 1
1 ILLUMINA (Illumina NovaSeq 6000) run: 58,547 spots, 15.2M bases, 5.1Mb downloads

Design: Illumina paired end sequencing of ribo-depleted total RNA, library prepared NEBNext Ultra II Directional RNA Kit
Submitted by: UConn Health
Study: Pyrococcus furiosus COM1 Raw sequence reads
PRJNA901420 • SRP408047 • All experiments • All runs
show Abstracthide Abstract
Pyrococcus furiosus MNase protected DNA was sequenced to determine patterns of protein binding to the chromosome, particularly near CRISPR arrays. Histones were identified as a key binding protein and were used in an in vitro integration study to determine their influence on CRISPR spacer integration. Both the in vitro integration sequencing data and the MNase-Seq data are available here.
Sample: Histone profile for Pyrococcus furious
SAMN31712712 • SRS15833182 • All experiments • All runs
Organism: Pyrococcus furiosus
Library:
Name: Pfu_WT_S1
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 58,547 spots, 15.2M bases, 5.1Mb
Run# of Spots# of BasesSizePublished
SRR2472503658,54715.2M5.1Mb2023-05-24

ID:
27926593

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